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KMID : 1001020050030010032
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Journal of Urologic Oncology
2005 Volume.3 No. 1 p.32 ~ p.38
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Effects of NF-κB on Human Bladder Tumor
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Jung Pil-Du
Lee Young-Suk
Park Moon-Seon
Jo Sung-Whan
Kim Wun-Jae
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Abstract
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Purpose: The transcription factor nuclear factor of kappa B(NF-κB) is activated in a broad range of human tumors and is thought to promote tumorigenesis, mainly due to its ability to protect transformed cells from apoptosis. In this study, we investigated whether the expression levels of NF-κB were related to the pathogenesis of human bladder tumor.
Materials and Methods: Fresh tissues of patients and controls were obtained during operation. Total RNA was extracted and cDNA was synthesized using reverse transcriptase. The mRNA levels of NF-κB were examined from 231 bladder tumor specimens, 67 corresponding normal looking bladder mucosae surrounding tumor and 22 normal bladder mucosae by real time PCR and the expression of NF-κB was investigated using immunohistochemical staining in 148 archival bladder tissue paraffin blocks including 136 transitional cell carcinomas of the urinary bladder and 12 normal bladder mucosae.
Results: The mRNA expression level of NF-κB in bladder tumor, corresponding normal bladder mucosae surrounding tumor and normal bladder mucosae was 32.221?2.036pg/ml, 3.060?0.305pg/ml and 0.907?0.131pg/ml, respectively. Real time PCR analysis showed that mRNA levels of NF-κB in bladder tumors were significantly higher than in both corresponding normal bladder mucosae surrounding tumor and normal bladder mucosae(p＜0.001). The tumor grade was significantly associated with the NF-κB status, since the NF-κB expressions were enhanced in low-grade tumors compared with high-grade on immunohistochemical staining(p= 0.015, OR=3.624, 95% CI=1.225-10.719). However, NF-κB expression was not associated with stage, recurrence, progression and patient’s survival.
Conclusions: Expression levels of NF-κB are strongly associated with the development of bladder tumor. But, NF-κB expression was not related to the clinicopathological findings. These results suggested that NF-κB might be activated during the initial stage of human bladder tumorigenesis.
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KEYWORD
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NF-κB, Real time PCR, Bladder tumor
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